psimple mirna302 367 Search Results


psimple mirna 302 367  (Addgene inc)
94
Addgene inc psimple mirna 302 367
Psimple Mirna 302 367, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/pm38712688-54-43-44?v=Addgene+inc
Average 94 stars, based on 1 article reviews
psimple mirna 302 367 - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
plasmids pep4 e02s en2l  (Addgene inc)
93
Addgene inc plasmids pep4 e02s en2l
Plasmids Pep4 E02s En2l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/bio_rxiv__64898__2026__03__17__712482-90-19-23?v=Addgene+inc
Average 93 stars, based on 1 article reviews
plasmids pep4 e02s en2l - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
pep4 e02s em2k  (Addgene inc)
94
Addgene inc pep4 e02s em2k
Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid <t>EM2K</t> was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.
Pep4 E02s Em2k, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/bio_rxiv__64898__2026__03__17__712482-90-30-33?v=Addgene+inc
Average 94 stars, based on 1 article reviews
pep4 e02s em2k - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
pcep4 m2l  (Addgene inc)
93
Addgene inc pcep4 m2l
Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid <t>EM2K</t> was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.
Pcep4 M2l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/pmc10724057__mmc1-56-26-34?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pcep4 m2l - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
sp6 ebna1  (Addgene inc)
94
Addgene inc sp6 ebna1
Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid <t>EM2K</t> was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.
Sp6 Ebna1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/pm40997543-38-54-55?v=Addgene+inc
Average 94 stars, based on 1 article reviews
sp6 ebna1 - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
pep4 e02set2k  (Addgene inc)
93
Addgene inc pep4 e02set2k
Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid <t>EM2K</t> was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.
Pep4 E02set2k, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/pmc10724057__mmc1-56-28-34?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pep4 e02set2k - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
pep4 160 e02sen2k  (Addgene inc)
93
Addgene inc pep4 160 e02sen2k
Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid <t>EM2K</t> was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.
Pep4 160 E02sen2k, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psimple+mirna302+367/pmc10724057__mmc1-56-23-34?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pep4 160 e02sen2k - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

Image Search Results


Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P16, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P12. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Article Snippet: In trials 7-13, fibroblast reprogramming was initiated by electroporating fibroblasts from W4, M4, M6, and W6 ( ) with plasmids pEP4 E02S EN2L (Addgene, plasmid # 20922), pEP4 E02S ET2K, pEP4 E02S EM2K (Addgene, plasmid # 20923), pSimple-miRNA302/367, and EBNA1 mRNA.

Techniques: Amplification, Sequencing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus

Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Journal: bioRxiv

Article Title: Replicable generation of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia

doi: 10.64898/2026.03.17.712482

Figure Lengend Snippet: Characterization of RhiPSC line M6 clone 16.02. (A) Amplicon sequencing of MAPT R406W with mutant T allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 µm. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1 , SOX2 , NANOG , KLF4 , LIN28 , and MYC . ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs at P18, no template control (NTC), and the rhesus embryonic stem cell line r420 (RhESC). Plasmid EM2K was used as a positive control (PC) for the detection of EBNA and oriP. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 µm. (G) Flow cytometry-detected APC-A+ cells were 93.1% positive for SOX2, 98% positive for OCT4, and 93.3% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed (+) RhiPSC line identity. Mycoplasma (Myco.), Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected (-). (I) Normal 42,XX karyotype at P15. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 µm.

Article Snippet: In trials 7-13, fibroblast reprogramming was initiated by electroporating fibroblasts from W4, M4, M6, and W6 ( ) with plasmids pEP4 E02S EN2L (Addgene, plasmid # 20922), pEP4 E02S ET2K, pEP4 E02S EM2K (Addgene, plasmid # 20923), pSimple-miRNA302/367, and EBNA1 mRNA.

Techniques: Amplification, Sequencing, Mutagenesis, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation, Positive Control, Immunofluorescence, Flow Cytometry, Virus